Protein Extraction and Gel Electrophoresis Lab Report

Protein Extraction and Gel Electrophoresis - Lab Report Example The sodium dodecyl sulfate coats the proteins in proportion to their molecular weight and then confers the same negative electrical charge across all proteins in the sample. The rate of migration of a polypeptide in SDS-PAGE is inversely proportional to the logarithm of its molecular weight. This means that the larger the polypeptide, the slower it migrates in a gel. The molecular weight is determined by comparing the migration of protein spots to the migration of standards. Plots of log molecular weight versus the migration distance are reasonably linear. The proteins separated by SDS-PAGE are often recovered in a procedure that involves localizing the protein of interest on the gel following SDS-PAGE, eluting the protein from the gel, removing the sodium dodecyl sulfate from the eluted sample, and finally renaturing the protein for subsequent analysis. Proteins that are eluted from gels are used in varied downstream applications successfully, such as protein chemistry, determinatio n of amino acid composition, identification of polypeptides that correspond to specific enzyme activity, and other purposes.The analysis of protein concentrations is a significant assay in biochemistry research. The Bradford assay is one of the most widely used methods to determine concentrations of protein, relative to a standard. The technique is based on the formation of a complex between proteins in solution and the dye. This assay is commended for overall use, particularly for assessing concentrations of proteins for gel electrophoresis.... The proteins separated by SDS-PAGE are often recovered in a procedure that involves localizing the protein of interest on the gel following SDS-PAGE, eluting the protein from the gel, removing the sodium dodecyl sulfate from the eluted sample, and finally renaturing the protein for subsequent analysis. Proteins that are eluted from gels are used in varied downstream applications successfully, such as protein chemistry, determination of amino acid composition, identification of polypeptides that correspond to specific enzyme activity, and other purposes. The analysis of protein concentrations is a significant assay in biochemistry research. The Bradford assay is one of the most widely used method to determine concentrations of protein, relative to a standard. The technique is based on the formation of a complex between proteins in solution and the dye, Brilliant Blue G. This assay is commended for overall use, particularly for assessing concentrations of proteins for gel electrophores is. It is based on observations that absorbance maximum for acidic mixtures of Coomassie Brilliant Blue G-250 that do shift from 465 nm up to 595 nm at a time when protein binding occurs. The assay is effective because of the extermination coefficient of the albumin-dye complex solution is usually constant over a range of 10-fold concentration (Westermeier, Naven & Ho?pker, 2008). The dye reacts mainly with arginine residues but less with histidine, lysine, tryptophan, tyrosine, and phenylalanine residues. Seemingly, this examination is not all that perfect for acidic or basic proteins. However, it is somewhat sensitive to the bovine serum albumin, even more than most proteins, by a factor of two. Gamma globulin (IgG) is the protein standard of preference. The objective of this

Ethical and Human Issues in Health Care Research Paper

Ethical and Human Issues in Health Care - Research Paper Example Health inequities generate issues that deeply question our moral convictions. Any society should provide its members with the requisite conditions for attaining the best possible health, as this leads to a contented and useful life. Such is the demand of justice in health. This viewpoint renders a global community unjust if it permits the premature demise of individuals. It should, in fact, promote the economic and social conditions that are essential for preserving life (Ruger, 2006, p. 1002). A segregation of the members of society on the basis of deprivation is to be achieved. This in turn clearly identifies the individuals who are in urgent need of access to health care. It is important to realize that this intervention does not abandon the improvement of the average health (Ruger, 2006, p. 1002). Moreover, the provision of medical facilities to the groups occupying the center of the ill health spectrum is not discarded in this endeavor. Consequently, universal health coverage is promoted with regard to diseases such as AIDS, malaria and tuberculosis (Ruger, 2006, p. 1002). These diseases have been seen to be prevalent to a much greater extent among the disadvantaged sections of society. Moreover, there is a close correlation between global health and health and human rights. This relationship is founded on the inherent value of every individual and the rights of a person that derive from the local and international community. It is the objective of global health to ensure these rights.

50 First Dates Essay Example for Free

50 First Dates Essay The movie 50 First Dates is a romantic comedy film directed by Peter Segal. The movie is a notion of a day that keeps repeating itself. However, in this movie the recycling takes place entirely inside the mind of Lucy Whitmore played by Drew Barrymore. Barrymore plays a young woman who was in an accident that caused her to suffer from Goldfield Syndrome, a fictional form of anterograde amnesia in which each day’s events disappear from her memory overnight. This is caused by damage to the hippocampus, inside the temporal lobe, as the hippocampus controls memory functions. Furthermore,she also innocently believes every day to be the birthday of her father which happens to be the day of the accident. In order to shield her from the pain of repeatedly learning about the accident, Marlin and Lucy’s brother, re-enact the activities of Marlin’s birthday every day. The character Oola is a friend of Sandler, whom helps come up with clever ideas to help Lucy fall in love with Henry everyday. Oola is affecting his neurotransmitters by the daily use of marijuana. Marijuana works as an agonist, in this movie. Adam Sandler, a womanizing marine life veterinian whom cares for animals in an aquatic amusement park has too many sexual conquests until he realizes that he is falling in love with Lucy. Sandler, sheds his philandering ways and devises new ways to meet her again every day, hoping that one day she will retain her memories and feelings for him. He eventually convinces Marlin that hiding the truth from Lucy is worse than explaining it to her each day, so they begin to leave video tapes for her to watch each morning, explaining her situation and her relationship with Henry. Lucy also writes notes to herself in a diary. Over time, she begins to reciprocate Henry’s feelings, even accepting his marriage proposal. Lucy is surrounded by a great deal of support and love throughout her dilemma. Lucy also receives deception from her brother and father and the basically the whole town because they protect her from knowing the truth. Personal Reflection: The results of this movie provide evidence that people suffer with anterograde amnesia which can effectively evaluate aspects of their quality of life and that the quality of communication life style. This movie also provides evidence that people with Amnesia also refers to an inability to recall information that is stored in memory. The causes of amnesia may be organic or functional. Organic causes may include brain damage through injury, or the use of specific drugs but usually sedative drugs. Amnesia may be one of the symptoms of some degenerative brain diseases, such as Alzheimer’s disease. Functional causes are psychological factors, such as defense mechanisms. People with amnesia also find it hard to imagine the future, because our constructions of future scenarios are closely linked to our recollections of past experiences. Researchers from Washington University in St. Louis used advanced brain imaging techniques to show that remembering the past and envisioning the future may go hand in hand with each process sparking strikingly similar patterns of activity within precisely the same broad network of brain regions. Fortunately, in the majority of cases amnesia resolves itself without treatment. However, if an underlying physical or mental disorder is present, it should be treated. Psychotherapy may sometimes be effective for some patients. Hypnosis can be an effective way of recalling memories that have been forgotten. Family support is crucial in helping a patient with amnesia get better. Psychologists and psychiatrists say that reality orientation aids may help to nudge patients back into their environment if they are surrounded with familiar objects, photographs, smells, and sounds. Personal Implications: In 50 First dates, it was designed to tell the story of Lucy a woman whom suffers from a severe memory loss and can not remembered anything that is happening but remembers the past. Goldfield’s syndrome is a fictive name for a specific form of Anterograde amnesia used in the romantic comedy. The condition causes Lucy to lose of all her memories of the day, save the memories prior to the accident, due to that she is unable to convert her short-term memories to her long-term memories during her sleep. This results in her waking up every day believing it is the day of the accident, October 13, 2002. So, while the name is fiction, it is based upon a real condition. The neurological condition that Lucy suffers from, Goldfield Syndrome, is entirely fictional. True anterograde amnesia affects either short-term memory, which can last minutes or seconds, or intermediate-term memory, which can last days or weeks. Although, falling asleep has nothing to do with the condition, and sleep actually intensifies many chemical effects which help memory.

Classifications of Government Expenditure

Classifications of Government Expenditure The welfare of the people of India highly depends on the expenditure of the Government of India (GOI). Government expenditure is a very important aspect of the governments budget presented by the finance minister every fiscal. Through it GOI tries to maximise the welfare of the people by appropriately allocating economic resources to various government activities. Government expenditure can be broadly classified into four categories: Functional Classification or Budget Classification: In April 1974, the GOI introduced a new accounting structure in order to serve the requirement of financial control and accountability. Under this structure, a five tier classification has been adopted sectoral, major head, minor head, subhead, and detailed heads of accounts. Sectoral classification has clubbed the government functions into three sectors, namely, General Services, Social and Community Services and Economic services. In the new structure, a major head is assigned to each function and minor head is assigned to each programme. Each minor head include activities or schemes or organizations as subheads. Economic Classification: Economic classification of the government expenditure signifies the way of the allocation of resources to various economic activities. It involves listing the government expenditure by significant economic categories, separating current expenditure from capital expenditure, spending for goods and services from transfers to individuals and institutions, inter-governmental loans from grants etc. This classification provides a record of governments influence in each sector of the economy. Cross Classification or Economic-cum-functional Classification: Cross classification provides the break-up of government expenditure both by economic and by functional heads. For example, expenditure on medical facilities, a functional head, is split as current expenditure, capital expenditure, transfers and loans. Accounting Classification: Accounting classification of the government expenditure can be presented under (i) Revenue and Capital (ii) Developmental and Non-Developmental and (iii) Plan and Non-Plan. Each classification of expenditure has an objective associated with it. For instance, Revenue and Capital expenditure indicates the creation of assets by government expenditure and unproductive expenditure. Further, the developmental and non-developmental classification differentiates the government classification as the expenditure on social and community services and economic service from that against general expenditure. Similarly, the Plan and Non-Plan expenditure represents the expenditure on planned schemes of government and non-plan expenditures. The above classification of the government expenditure serves one or more of the purposes of the government, such as, parliamentary control over expenditure, economic development, price stability etc. Analysis of Government Expenditure in India The chart given below shows the division of government expenditure in terms of capital and revenue expenditure for some selected years. The percentage share of revenue expenditure in government expenditure has increased over time due to increase in expenditure in society welfare. The growth story of the India can be said in terms of five year plans which give an account of government expenditure during the plans. The first five year plan (1951-56) laid emphasis on agriculture, including irrigation and power, wherein the government had spent 36% of its subject on these heads. The second five year plan (1956-61) marks the foray towards industrialization with an increased government spending in transport and communication sector with an contribution of 28.9% of its expenditure. The urge for industrialization continues even during the third five year pan (1961-66) with 24% and 20% public spending on Industry Minerals, and Transport and Communication respectively. Fourth to seventh five year plans have social services of education, health, welfare sharing a majority of the pie with 24% and 26% expenditure in fourth and fifth plan. The eight five year plan (1992-97) commenced the era of fiscal reform and liberalization. There were increased efforts to improve the economic growth and quality of life of the common man. There were high public spending on the sectors like energy (26.6%), Transportation and communication (20.8%) and Social and other services (19.6%). The Ninth five year plan (1997-2002) focussed on the development of infrastructure by allocating 72% of the funds to irrigation, energy, transport and communication and social service. The tenth five year had an objective of atleast 8% growth rate by providing a boast to power sector spending (26.56%) and increasing social Community services sector spending to 29.27%. COMPARISON OF INFLATION BASED ON CPI AND WPI Consumer Price Index (CPI), is defined as the weighted average change over time in the prices of a basket of the good and services consumed by a consumer. While, Wholesale Price Index is defined as the weighted average change over time in the price of a basket of wholesale goods. Inflation rate is calculated based on the CPI and WPI as the percentage in the respective indices over time, generally a year. The calculation of inflation rates based on CPI and WPI form 1994-95 to 2006-07 is given in the table provided below: CPI and WPI differ in terms of their weights assigned to their respective constituent basket goods and services. Food has been assigned a higher weight in CPI (46% in CPI-IW) as compared to a weight of only 27% in WPI. As a result, the CPI changes more with the change in the food process as compared to the WPI. Further, the fuel group has a much lower weight in CPI (7% on an average) relative to that in the WPI (14.2%). Consequently, the variations in international crude process affect the WPI more than CPI. Also, services are not included under WPI, but are included in the CPI to different extents. Hence, the CPIs are influenced by the service price inflation. Considering the data provided in the table above, it can be inferred that on an average CPI-IW inflation is higher than that of WPI inflation. Also, in terms of WPI inflation the period from 1994-95 to 1995-94 was of higher inflation, then the inflation decreases and increases again in 2000-01 and then decreases again. It can be seen that the inflation rate, both CPI inflation and WPI inflation, follows a cyclic pattern.

The World Needs a Little Courtesy Essay -- Argumentative Persuasive Es

The World Needs a Little Courtesy Doesn't anyone show common courtesy anymore? When I was younger, I never knew what my parents meant when I heard them ask that question. I thought people were polite. I never had any problems with rude people. Now that I am older, and actually experience people outside of school, I understand what my parents meant. What happened to people saying "Excuse me" when they want to walk past you? Don't people know what it means when someone says this to them? When I say "Excuse me" to someone the individual stares at me as if I just spoke a foreign language or said something insulting. Then the individual still doesn't move. I don't know how many times I have been standing somewhere, and instead of someone saying "Excuse me" to get by, he or she just shoves past. How was I to know the person was standing behind me? I guess I should have used the eyes in the back of my head. I have also seen grown men push past pregnant women, elderly couples, and even handicapped people as if they weren't even there. When my boyfriend was on crutches and we went shopping, people would run into him with their carts. He had to stop and get out of their way and they would act as if it was an inconvenience to them that he was on crutches. I look out for pregnant women, the elderly, people on crutches or in wheelchairs, someone with his or her arms full, and people with children. I move out of their way. Am I just more aware of my surroundings than other people? What happened to holding doors open for other people? I don't mean for just women, the elderly, or the handicapped. (I am a twenty-five-year-old female,... ...ould be because parents do not have the support of the teachers and schools. Teachers are a big part of a child's life, but they are not allowed to correct a child's behavior because they could be reprimanded for doing so. If we do not teach our children to respect their elders, to help others, and to say please and thank you, then eventually common courtesy and politeness will be lost. Did you ever realize how a reaction you get from a stranger, or even someone you know, could affect the mood of your day? Or how polite or rude that person was? Did you ever feel a little happier after doing something nice for someone? For most of us, our days are filled with stress. Being courteous to someone or smiling or saying "Thank you" might just change both of your moods. Try it sometime. It might just change your whole day. Thank you!

Development of Attachment Essay -- Psychology

It has been shown that the relationships infants develop early on in life have lasting effects on their identity and behavior. Extensive research has indicated that the relationship between an infant and its caregivers is particularly important. All children are different, and in order to have a healthy relationship with your child, you should adapt your parenting methods to fit his specific needs. All children differin fundamental ways, two of the most comprehensive being their temperaments and attachment styles. A child’s temperament is the way in which he reacts to the world, new situations, people, and experiences. Attachment is an infant’s enduring emotional bond to his parentsor primary caregivers. Both of these factors affect children not only in infancy, but throughout their lives. Temperament has been shown to be a consistent aspect of a  person’s behavior over time, and their style of attachment to their primary caregiver often shapes the quality of platonic and romantic relationships with others as they age. A child’s temperament can affect his parent’s reactions and feelings toward him, and subsequently his attachment style. Infants are put into three defined categories of temperament: easy, difficult, and slow to warm up. Easy babies adjust readily to new experiences and are generally happy and easy to calm. Difficult babies are easily upset, have intense negative emotional reactions, and have irregular bodily functions. Slow to warm up babies react to new stimuli as difficult babies would initially, but with repeated exposure will react more like easy babies. Easy babies often engender positive reactions and feelings from their parents, which helps produce a secure attachment relationship between the paren... ...t them independently. This not only sets him up for immediate success in school or whatever he is focusing on, but also in the future when dealing with college or work. He will be more motivated to complete his work to the best of  his ability and to work past any barriers or conflicts that arise. Works Cited Allard, Lindsey T., and Amy Hunter. â€Å"Understanding Temperament in Infants and Toddlers.†Ã‚  Center on the Social and Emotional Foundations for Early Learning. Vanderbilt University, n.d. Web. 17 Nov. 2014. . Davis, Jeanie L. â€Å"10 Commandments of Good Parenting.†Ã‚  WebMD. N.p., n.d. Web. 17 Nov. 2014. . Siegler, Robret, Judy DeLoache, and Nancy Eisenberg.  How Children Develop. 3rd ed. New York: Worth Publishers, 2011. 425-98. Print.

Immanuel Kant Paper Essay

Immanuel Kant (1724-1804) is one of the most influential philosophers in history of Western philosophy. A main representative of the Western-European classical philosophy, Immanuel Kant dealt with the best traditions of the German idealism. A human personality, according to Kant is the highest and absolute value. It is the personality, in Kant’s understanding, that towers the person over its own self and links the human being with the â€Å"order of things†. The â€Å"order of things†, according to Kant is the reflection of the â€Å"common sense†. The whole perceived world around us complies with the â€Å"order of things†. The most interesting part of Kant’s philosophy is that his own notion of the â€Å"order of things† and â€Å"common sense† is dual in case of analyzing it. In his work â€Å"Groundwork of the Metaphysic of Morals† Kant’s thoughts come to a vital question. This question lies in the fight between the undermost and highest abilities of a desire, between the longing to happiness and a good will that is a manifestation of duty. In this work it is very easy to trace Kant’s intension to oppose the â€Å"clean† teaching of morality and its degradation to any ethical relativism, which causes this degradation of morality. He tried to create the highest form of estimation of the ethical principles that managed morality? For he believed that the degradation of morality starts from, the impossibility to evaluate what is really wrong or right. Here, is the point where Immanuel Kant enters the definition of the notion â€Å"good will†. Kant explains what a â€Å"good will† is and what difference it makes in the perception of the moral actions that a person makes. Kant’s main intention is to underline the moral value of motivation and its realization at least through the presence of a â€Å"good will† in a person. Kant 3 Analysis of Kant’s â€Å"good will† â€Å"The only thing that is good without qualification or restriction is a good will†. Kant outlines that goodness objectively remains to be goodness, even if nobody is really good. From this understanding he offers the definition of what a good will is. The notion of Kant’s ethics is the autonomous good will. This â€Å"good will† is not passive; a carrier of this will needs to make actions and deeds. A moral action looks like a result of an internal imperative command. So Kant makes an accent on the importance of the behavior, which is managed by the good will. â€Å"That is to say, a good will alone in good in all circumstances and in that sense is an absolute or unconditioned good†. Kant evaluates a good will without basing on the results, which it produces. From the point of view of the philosopher a good will keeps remaining good even if it does achieve the goal it had. In other words, even if an individual fails to do something commanded by his good will what he did remains a good thing unconditionally. At the same time Immanuel Kant views a good will as a unique goodness that is able to produce the result it intended to produce. A good will is still valuable by itself, because it objectively either exists or not in the personality. â€Å"Good will† vs. Happiness As it has been already mentioned Immanuel Kant in his work â€Å"Groundwork of the Metaphysic of Morals† widely uses the term â€Å"good will†. It is very important to understand the Kant 4 reason Kant valued this notion so deeply. To completely understand this it is necessary to draw the parallel between a good will and objective happiness of every individual. Let us suppose, basing on Kant’s words, that we meet a person who at all points is successful: he has power, wealth, a good health, a good state of mind, he is satisfied with his life, looks and considers himself to be a happy person. Can we say analyzing this man that he is happy? Generally yes. People would ordinarily say that this man is happy and has everything to be happy and his own perception of being happy in addition. Kant’s question to this matter is different – does this happiness have a moral base? From Kant’s point of view some conditions and qualities of a â€Å"happy person† are not combined with any moral bases. At this point is necessary to come back to the term â€Å"good will†. The absence of a good will makes unacceptable generally needed personal qualities such as wittiness, ability to judge, courage, decisiveness and many others. Kant implies that these qualities may become â€Å"evil† in case when they are not supported by the good will. From the philosopher’s opinion a good will forms, probably, the most essential condition not only of being happy but even of being worthy to be happy. The essence of a â€Å"good will† A â€Å"good will† is a will, not able to be cruel or evil. The â€Å"supposition† of goodness forms the nature of the good will. Goodness it the main requirement of the existence of the â€Å"good will† according to Immanuel Kant. A good will is a will in which subjective characteristics of an individual do not prevent but define and help the â€Å"desire to do good†. Good will in its own sense is a unity of liberty and law, mind and goodness. The purity of determination of the will by the Kant 5 mind is the real meaning of its goodness. Kant also refers to the â€Å"absolute good will†. The â€Å"sanctity† of this good will in Kant’s understanding comes from its superiority over Kant’s ordinary †good will†. Kant views the absolute good will as the moral destination of the human mind. It is the main goal of the highest gift humanity has ever gotten – the human mind. Kant interprets this form of will as a â€Å"pure† will. Kant through his notion â€Å"good will† reveals the necessity of high moral values in the life of every person, without which the life even subjectively happy individual is may not be called complete and successful, but even a failure of mind to success and to purify. Kant makes an accent on the required versatility of the good will –a good will needs to become the law of will of any creature. A good will possesses a full unity of subjective and objective beginnings of the will in the unconditional law of moralities. Plausibility of Kant’s view of the â€Å"good will† Some people claim that Kant’s idea seems to be a utopia of perfection. According to Kant the perfection of will is estimated by the completeness of absolute will in an individual. If therefore the perfection of will is a moral law, all other laws of duty remain considerably imperfect, which also makes the good will imperfect, too. Therefore, a human being is a unique creature, for which perfection in other fields is not as valid, as the good will is. The complete perfection is achieved only the good will itself, for it is objectively and unconditionally good. Can this be considered to be a universal law? And is a good will the most valuable thing philosophically? What Kant assures the reader is that the good will is good even if it does not produce the necessary effects and does not achieve the results is aimed to achieve. If the reader analyzes this point of view from a practical example he might face a certain difficulty. For instance, a person’s Kant 6 goal is to do a good thing, but while trying to do it he causes definite harm. Can it be estimated to be still a good will, even if it caused negative results, opposite to the ones aimed to achieve. The most questionable part of Kant’s opinion is the evaluation of the result of the action produced by the good will. As both positive and negative results do not postpone the goodness of the good will it is very hard to objectively judge the actions of a person. If the perfection can be found only inside of the good will and nothing else can be perfect by itself, than how can a person assume that he poses a good will. This is very doubtful, due to the difference of the notion of happiness of other people. Individual’s subjectivity does not allow them to possess a good will because even if their intensions are good they cannot know for sure that what they want to do is good in terms of the person they want to help and anything else. At the same time the fact of willing good may not be taken into count. If there are two different people with the same results obtained and if their wills are opposing each other, than the person that possessed a good will in his intentions is the â€Å"good† one. It is about the inconsistency of the will and the result of the action the will produces. Kant idea seems to be a utopia, but nevertheless, it may be called plausible due to the possibility to perfect while trying to achieve the absolute will. It is very plausible that even if a man is talented, gifted, if he posses the most suitable temperament for his ambience, even if he is clever, voluptuous, sincere it all may produce harm in case if it not directed by a good will. Immanuel Kant outlines that a good will is what makes a man highly moral and therefore evaluates him in its own definition. Conclusion Immanuel Kant provided a philosophical point of view of the morality of the society. His â€Å"good will† innovation gave the bases to the works of other famous philosophers. Immanuel Kant 7 Kant’s â€Å"good will theory† claims that only if a thing does not require anything else to be good in order to function – then it is good and if it does it is good with a certain limitation. â€Å"Good will† is estimated both by the goodness of the willing and the result of this willing, not necessarily both at the same time. As all the personal qualities require a good will to conduct them in order to be good – they are all good with limitations. At the same time a good will does not need all these qualities to be good. All the qualities and opportunities in the society around are good with limitations and the only thing that will make them truly good – is the good will, for it is the only thing that does not require anything else to be good. Kant’s unexcelled idea enraptures the thinker with its depth. The most important thing to understand, according to Kant is that the goodness of a good will is not evaluated through the result it achieves and the result itself has nothing to with the goodness of the good will and under no condition does limit its goodness. Nothing would end this paper better that Kant’s own words carrying the most important message he wanted people to hear: â€Å"It is impossible to conceive anything at all in the world, or even out of it, which can be taken as good without qualification, except a good will†. Kant 8 Bibliography Abbott, I. K. (1829). Groundwork for the Metaphysics of Morals. New York: Broadview Press Ltd. Beck, t. L. (1959). Foundations of Metaphysics of Morals. Indianapolis: Bobbs-Merrill. Kessler. (2009). Voices of Wisdom. Mason: Cengage Learning.